Next-Generation Sequencing (NGS): new technologies to access microbial communities
DOI:
https://doi.org/10.35642/rm.v8i1.1248Keywords:
Sequencing, Technologies, Microorganisms, Diversity, DataAbstract
Next-Generation Sequencing (NGS) are platforms capable of generating millions of DNA or RNA sequence data in a single round through in vitro cloning, a technique used to amplify the number of molecules for analysis. However, it is necessary to gather information that enables researchers to determine the best methodology for their work. Therefore, the objective of this article is to conduct a literature review on NGS technologies, comparing them with each other and with more traditional methods, highlighting their importance in agricultural and environmental microbiology. The Sanger method is currently the most well-known sequencing technique. However, NGS platforms employ methodologies that streamline sequencing and the advancement in these techniques have significantly reduced sequencing costs. To carry out the studies, data from articles and platforms developed by companies that own these technologies were used to perform comparisons and analyses on the topic. Therefore, this manuscript is a bibliographic research and characterized as review article. While several second-generation sequencing platforms are available on the market, most are expensive and restricted to a few countries. There is still difficulty in finding suitable algorithms to analyze the amount of data generated. Nevertheless, NGS methods have generated good expectations and promise important advancements in studies aimed at accessing and identifying the groups that make up the microbial community structure in environmental research.
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